An (R)-Selective Transaminase From Thermomyces stellatus: Stabilizing the Tetrameric Form
نویسندگان
چکیده
منابع مشابه
Crystal Structure of an (R)-Selective ω-Transaminase from Aspergillus terreus
Chiral amines are important building blocks for the synthesis of pharmaceutical products, fine chemicals, and agrochemicals. ω-Transaminases are able to directly synthesize enantiopure chiral amines by catalysing the transfer of an amino group from a primary amino donor to a carbonyl acceptor with pyridoxal 5'-phosphate (PLP) as cofactor. In nature, (S)-selective amine transaminases are more ab...
متن کاملThe substrate specificity, enantioselectivity and structure of the (R)-selective amine : pyruvate transaminase from Nectria haematococca
UNLABELLED During the last decade the use of transaminases for the production of pharmaceutical and fine chemical intermediates has attracted a great deal of attention. Transaminases are versatile biocatalysts for the efficient production of amine intermediates and many have (S)-enantiospecificity. Transaminases with (R)-specificity are needed to expand the applications of these enzymes in bioc...
متن کاملCrystallization and preliminary X-ray diffraction studies of the (R)-selective amine transaminase from Aspergillus fumigatus.
The (R)-selective amine transaminase from Aspergillus fumigatus was expressed in Escherichia coli and purified to homogeneity. Bright yellow crystals appeared while storing the concentrated solution in the refrigerator and belonged to space group C222(1). X-ray diffraction data were collected to 1.27 Å resolution, as well as an anomalous data set to 1.84 Å resolution that was suitable for S-SAD...
متن کاملPhenolics from Rhagadiolus stellatus (Asteraceae, Cichorieae)
Rhagadiolus stellatus Gaertn., a Mediterranean member of the Cichorieae tribe of the Asteraceae family used as a food plant, was analyzed for its spectrum of phenolic compounds. Kaempferol 3-O-β-glucoside 1, kaempferol 3-O-β-rutinoside (nicotiflorin) 2, quercetin 3-O-β-glucoside 3, and luteolin 4 were isolated from the n-butanol layer of a methanolic extract of whole plants of Rh. stellatus of ...
متن کاملStabilizing an atom laser using spatially selective pumping and feedback
We perform a comprehensive study of stability of a pumped atom laser in the presence of pumping, damping, and outcoupling. We also introduce a realistic feedback scheme to improve stability by extracting energy from the condensate and determine its effectiveness. We find that while the feedback scheme is highly efficient in reducing condensate fluctuations, it usually does not alter the stabili...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Frontiers in Bioengineering and Biotechnology
سال: 2020
ISSN: 2296-4185
DOI: 10.3389/fbioe.2020.00707